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stempro-34 sfm (1x)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stempro-34 sfm (1x)
    Stempro 34 Sfm (1x), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stempro-34+sfm/stempro+34+sfm/bio_rxiv__2025__07__08__663334-181-8-12
    Average 90 stars, based on 1 article reviews
    stempro-34 sfm (1x) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Membrane:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Injection:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Blocking Assay:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Cell Culture:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Pore Size:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Sterility:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Lysis:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Staining:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Luciferase:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Cell Differentiation:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Concentration Assay:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Virus:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Knock-Out:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).


    Gentle:

    Article Title: Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment
    Article Snippet: StemPro-34 SFM (1X) , Thermo Fisher Scientific , Cat#10639-011.

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PAN-Biotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: Impaired primitive erythropoiesis and defective vascular development in Trim71-KO embryos.
    Article Snippet: Differentiation of mESC was performed as embryoid bodies (EBs) in suspension by the removal of 2i and culturing in differentiation medium: StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 10% FCS, penicillin–streptomycin, 2 mM L-glutamine (PANBiotech), 40 μg/ml apotransferrin (Merck), 0.5 mM L-ascorbic acid (Sigma-Aldrich), and 0.15 mM 1-MTG (Sigma-Aldrich).

    Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
    Article Snippet: CD45.2 BM cells were plated at 1 × 10 6 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (ThermoFisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine, 50 IU/ml penicillin, 50 μg/ml streptomycin and 55 μM β-mercaptoethanol.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: Briefly, PBMCs were cultured in StemPro-34 SFM (ThermoFisher Scientific, Waltham, MA, USA) and supplemented with SCF, FLT-3, IL-3, and IL-6 (Peprotech, Cranbury, NJ, USA).

    Article Title: A polystyrene-film-based device for engineered cardiac tissues enables accurate analysis of drug responses on contractile properties.
    Article Snippet: The EBs were subsequently cultured in a culture medium composed of StemPro-34 SFM (Thermo Fisher Science) supplemented with 2 mM L-glutamine (Invitrogen), 0.4 mM monothioglycerol (Sigma), 50 μg mL−1 ascorbic acid (Sigma), 150 μg mL−1 transferrin (Wako), and 5 ng mL−1 VEGF.

    Article Title: MiR-221-3p Attenuates IL-33-Induced Mast Cell Cytokine Expression by Targeting KIT.
    Article Snippet: The cells were cultured in StemPro-34 SFM (Thermo Fisher Scientific) containing 1 × GlutaMAX (Gibco), 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco).




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